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[Objective] The aim was to study on the soil nutrient index of Schisandra chinensis (Turcz) Baoll planting base in Yanbian area, so as to provide basis for its scientific fertilization. [Method] Twelve nutrient indeices of 20 soil samples from the planting base in Yanbian area were determined. Through the principal component analysis on these twelve nutrient indices, six main indices, including total N, total P, organic matter, available P, total K and total Ca, were studied by cluster analysis. [Result] The planting soil of S. chinensis in Yanbian could be classified into three types. There were 4 samples in the first type, 6 in the second, and 10 in the third. And 50% of the soil samples were classified into the first type. [Conclusion] Soil for planting S. chinensis showed strong acid with high content of available P, as well as relatively high contents of total N, total K, available B, available Mn, available Fe and organic matter. But available Cu content was greatly low in 50% of the soil samples; and Zn was lacked in 10% samples. Thus, a balanced and reasonable fertilization was recommended to increase the fertilizer efficiency.
2011年06期 v.2 1-4+8页 [查看摘要][在线阅读][下载 140K] <正>Through the joint efforts of in Editorial Oepartment and multitudinous readers, Medicinal Plant eventually passes the extremely strict appraisal, to be embodied by CABI's full text repository.Since 2010, Medicinal Plant has also been embodied by Chemical Abstracts (CA) and The Agricultural Sciences and Technology (AGRIS).
2011年06期 v.2 2页 [查看摘要][在线阅读][下载 231K] -
[Objective] The aim was to compare the quality of different cultivars of Citrus grandis var. tamentosa Hart.[Method] Characteristics of different varieties of C. grandis were observed and described, and the contents of total flavonoid, naringin and rhoifolin were determined by UV and HPLC. And genetic distance among different variations was analyzed by AFLP. [Result] Phenotype characteristics varied greatly among the cultivars of C. grandis. Contents of total flavonoids and naringin were much more higher in Dachaling and Fengwei than in other cultivars, and rhoifolin content was the highest in Dachaling. Genetic similarity among different cultivars was greater than 95%. [Conclusion] Phenotypic variation of different cultivars of C. grandis had basically taken shape, but that of genotype was still not yet formed. The qualities of Dachaling and Fengwei were the best.
2011年06期 v.2 5-8页 [查看摘要][在线阅读][下载 363K] [Objective] The aim was to study on the biological yields of different parts of Curcuma phaeocaulis Val. and to carry out preliminary study on different resource quality characteristics, so as to provide scientific basis for the development and utilization of its stems and leaves resources. [Method] Representative plants of Curcuma spp. in main producing region were selected randomly and the different parts of plants were taken as the tested materials. According to the methods in 2005 Chinese Pharmacopoeia, plant yields and quality characteristics of C. phaeocaulis were detected. [Result] Yield of Ermujiang per hole reached 224.44 g; photosynthates in stems and leaves, fat-soluble ingredients, protein yields accounted for 40.61%, 42.05% and 38.06% of the total resources, respectively. The content of fat-soluble ingredients in leaves was the highest (2.897%). The total ash content and acid-insoluble ash content in C. phaeocaulis met the demand for RHIZOMA CURCUMAE and Curcuma spp. in the 2005 Chinese Pharmacopoeia. [Conclusion] Stems and leaves of Curcuma phaeocaulis Val. were available and valuable resources. Therefore, C. phaeocaulis in main production area should be harvested before wilting; and the stems and leaves of C. phaeocaulis should be reutilized to enhance its oil yield, or be used as feedstuff, which could further enhance the economic benefit of peasant households.
2011年06期 v.2 9-11页 [查看摘要][在线阅读][下载 84K] -
[Objective] To explore the inhibitory effect of three antimicrobial agents against Aspergillus niger, a dominant endophytic fungus in Huperzia serrata (Thunb) Trev. [Method] Aspergillus niger was isolated from Huperzia serrata (Thunb) Trev by plate coating method, and the inhibitive effect of three antimicrobial agents against Aspergillus niger was obtained by mycelial growth rate method. Effect of three antimicrobial agents on H. serrata was conducted by inoculating stem segments on MS media with antimicrobial agents or by inoculating stem segments which were soaked in the minimum concentrations of antimicrobial agents for one or two days on MS media without antimicrobial agents. [Result] The minimal inhibitory concentrations of amphotericin B, sodium benzoate and cinnamaldehyde for Aspergillus niger were 512.000 00 mg/L, 4.00 g/L and 0.500 000 mg/L, respectively. Their EC50 were respectively 1.910 00, 150.000 00 and 0.120 00 mg/L, and their EC90 were 88.22, 730.00 and 0.330 00 mg/L respectively. Amphotericin B was the optimum antimicrobial agent for Aspergillus niger in Huperzia serrata (Thunb) Trev. [Conclusion] The minimum inhibitory concentrations of three antimicrobial agents for Aspergillus niger were obtained, which provided experimental basis for tissue culture of Huperzia serrata (Thunb) Trev.
2011年06期 v.2 12-14页 [查看摘要][在线阅读][下载 84K] [Objective] The aim was to study on the absorption and distribution laws of potassium in RADIX HEDYSARI and its response to fertilizer application, so as to provide theoretical basis for construction of GAP base. [Method] Random block experimental design was used to study on the effects of the application proportion of palygorskite and N-P-K fertilizer on the absorption and distribution of potassium in RADIX HEDYSARI. [Result] The potassium rapid accumulation period in the aboveground part of RADIX HEDYSARI was from late July to late August; the potassium rapid accumulation period in the root appeared from late August to late September. Combined application of palygorskite and N-P-K could enhance the potassium content in root, and the potassium accumulation in root and aboveground part of RADIX HEDYSARI. Palygorskite application had extremely significant binomial relationship with the agronomic efficiency of potassium fertilizer, and had linear correlation with the use efficiency of potassium fertilizer in RADIX HEDYSARI. [Conclusion] Palygorskite with the dose of 2 250 kg/hm2 could significantly promote the yield-increasing effect of potassium fertilizer and the utilization ratio of potassium fertilizer in RADIX HEDYSARI.
2011年06期 v.2 15-18+21页 [查看摘要][在线阅读][下载 163K] -
[Objective] The aim was to study the correlation among several quantitative characters of Prinsepia utilis Royle. [Method] Taking P. utilis in Shangbaimai Village, Daju County, Lijiang City as tested materials, correlation analysis, path analysis and principal component analysis of the quantitative characters were conducted, in order to find out the quantitative characters directly affected and had close correlation with the yield formation. [Result] The yield of P. utilis was closely related to crown width and fruit number per inflorescence, among which crown width affected the yield per plant mainly through fruit number per plant. Therefore, the relationship between crown width and fruit number per inflorescence should be coordinated during production. Besides, there was positive correlation between single fruit weight and yield per plant, which should also be paid attention during production. [Conclusion] This research provided references for the high-yield cultivation and breeding work of P. utilis in future.
2011年06期 v.2 19-21页 [查看摘要][在线阅读][下载 86K] [Objective] To study antioxidation of tannin from pomegranate rind and green tea. [Method] Antioxidation of tannin was studied in terms of OH· clearance rate, O-2 clearance rate and antioxidant activity on fat. [Result] The free radical clearance capacities were higher in green tea than that in pomegranate rind. The tannin from green tea had strong antioxidant ability on unsaturated fatty acids and weak antioxidant ability on saturated fatty acid, and the tannin from pomegranate rind was on the contrary. [Conclusion] The tannin from green tea was more suitable for research in healthy and beauty world.
2011年06期 v.2 22-24页 [查看摘要][在线阅读][下载 154K] -
[Objective] To investigate the effects of peimisine on developmental toxicity by the system of embryonic stem cells (ESCs) test. [Method] RT-PCR method was utilized to study the influence of peimisine at different concentrations of 0, 20, 40, 60 and 80 μg/ml on ESCs differentiating into cardiocytes with MHC specific expression after hanging drop and suspending culture and neuron cells with nestin specific expression after inducing by 5.0×10-7 mol/L retinoic acid. Effects of peimisine on cells viability of ESCs and BALBC(3T3) were assessed by alamar blue. Combined with the inhibitory effects on cells viability, the developmental toxicity characteristics of peimisine could be evaluated clearly. [Result] The inhibition concentration was the concentration of peimisine that could differentiate half ESCs into cardiocytes (ID50 D3 MHC) or neuron cells (ID50 D3 nestin) in vitro. The ID50 D3 MHC and ID50 D3 nestin of peimisine were 94.8 and 110.7 μg/ml, respectively. [Conclusion] Peimisine was identified as none-embryotoxicity and none-developmental neurotoxicity.
2011年06期 v.2 25-28+32页 [查看摘要][在线阅读][下载 166K] [Objective] The aim was to study on the bacteriostasis and antioxidation of the different solvent extracts from FLOS CARYOPHYLLI and CORTEX MAGNOLIAE OFFICINALIS. [Method] The ethanol extracts from FLOS CARYOPHYLLI and CORTEX MAGNOLIAE OFFICINALIS were extracted by petroleum ether and ethyl acetate, respectively. Eight common strains were used to evaluate the antimicrobial activity of the extracts by filter paper method; and the antioxidation activities of the extracts were determined, respectively, by DPPH method and Rancimat method. [Result] Petroleum ether extract from FLOS CARYOPHYLLI, petroleum ether extract and ethyl acetate extract from CORTEX MAGNOLIAE OFFICINALIS had relatively strong bactericidal capacity to all the tested strains. Extracts from FLOS CARYOPHYLLI had relatively strong scavenging capacity to DPPH free radical, while those from CORTEX MAGNOLIAE OFFICINALIS were relatively weak. The antioxidant activities of extracting solutions in lard system were in the order of ethyl acetate extract from CORTEX MAGNOLIAE OFFICINALIS>petroleum ether extract from CORTEX MAGNOLIAE OFFICINALIS>ethyl acetate extract from FLOS CARYOPHYLLI>petroleum ether extract from FLOS CARYOPHYLLI>water extract from FLOS CARYOPHYLLI>water extract from CORTEX MAGNOLIAE OFFICINALIS. [Conclusion] This research provided scientific basis for exploring natural food preservatives and antioxidants.
2011年06期 v.2 29-32页 [查看摘要][在线阅读][下载 180K] -
[Objective] The aim was to study on the effects of polysaccharides extracted from Dendrobinm chryseum Rolfe on the proliferation of mice spleen lymphocytes and peritoneal macrophages. [Method] The mice spleen lymphocytes and peritoneal macrophages were cultured in vitro and treated with or without polysaccharides. The changes in proliferations of these immune cells were detected by MTT. [Result] Medium and high concentrations of polysaccharides could significantly increase the proliferation of mice spleen lymphocytes and peritoneal macrophages, while the low concentration one had no enhancement effect. [Conclusion] Polysaccharides could promote the proliferation of mice spleen lymphocytes and peritoneal macrophages in vitro, indicating that it was able to enhance the immunity of mice.
2011年06期 v.2 33-34+37页 [查看摘要][在线阅读][下载 171K] [Objective] To explore the scavenging activities on DPPH free radical of bioflavonoid of kidney beans under different germination periods. [Method] After temperature-keeping and humidity-keeping germinating of kidney beans seeds, 1, 3, 5, 7 and 9 day-old germinating seeds were taken and dried then extracted with ethanol for preparing bioflavonoid extracts under different germination periods whose DPPH scavenging activities were also investigated. [Result] The bioflavonoid content of kidney beans decreased after germination, and the lesser of the content, the longer of the germination time; the scavenging activities on DPPH free radical tended to decrease with the increase of germination time, which also decreased with the increasing dilution degrees under the same germination period; DPPH scavenging rates of bioflavonoid extracts made under different germination periods were far bigger than the vitamin C of the same concentration. [Conclusion] The bioflavonoid of kidney beans had stronger capability to scavenge the free radical.
2011年06期 v.2 35-37页 [查看摘要][在线阅读][下载 144K] -
[Objective] This study to investigate the inhibitory effects of mycelium polysaccharide from Ganoderma lucidum Karst and Cordyceps sobolifera (Hill)Berk. et Br. on mouse Lewis lung cancer. [Method] For each healthy rat tested, 0.2 ml of lung cancer suspension was inoculated at the axilla. The rats were medicated with mycelium polysaccharides from G. lucidum and C. sobolifera for continuous 9 d by intragastric administration for measuring the indices of thymus and spleen and the tumor inhibition rate, thus establishing the lung cancer suspension model. [Result] At the dose of 200 mg/kg·d, both G. lucidum and C. sobolifera reached the optimal inhibition rates, of 50.2% and 46.3% respectively. [Conclusion] Mycelium polysaccharides from G. lucidum and C. sobolifera are endowed with inhibitory effects on Lewis lung cancer, and the former one is superior to the latter.
2011年06期 v.2 38-40+42页 [查看摘要][在线阅读][下载 197K] [Objective] The aim was to observe the effects of total saponins from Ranunculus japonicus Thunb. on hypoxia tolerance and memory ability of mice. [Method] Mice were given with different doses of total saponins from Ranunculus japonicus Thunb. by gastric perfusion for successive 14 days. Then, the hypoxia tolerance time and successful times of mice to run a maze were calculated, respectively. [Result] Mice treated with low-dose and high-dose total saponins from Ranunculus japonicus Thunb. could significantly prolong anti-hypoxia time and improve memory ability. [Conclusion] The total saponins from Ranunculus japonicus Thunb. could significantly promote intelligence development and prolong life of mice. Thus, total saponin is of high medical value and has good development prospect.
2011年06期 v.2 41-42页 [查看摘要][在线阅读][下载 75K] -
[Objective] The aim was to explore antioxidation of proanthocyanidin from pine needles in the serum of mice, so as to provide reliable experimental support for its further development and utilization. [Method] Effects of different concentrations of proanthocyanidin from pine needles on SOD activity, MDA content and organ indexes of mice were compared through gastric perfusion. [Result] Compared with blank control group, SOD activity and MDA content of mice increased and decreased significantly (P<0.05) in treatment groups, respectively; while there was no significant difference in viscera indices of mice among treatment groups. [Conclusion] Proanthocyanidin from pine needles had antioxidative activity to a certain extent.
2011年06期 v.2 43-44+46页 [查看摘要][在线阅读][下载 141K] [Objective] The aim was to determine the puerarin and daidzin contents in total flavonoids extracts of RADIX PUERARIAE. [Method] HPLC conditions were: chromatographic column of C18 column, mobile phase of acetonitrile-water (25∶75, V/V), detection wavelength of 250 nm, flow speed of 0.8 ml/min, column temperature of room temperature and sampling amount of 4 μl. [Results] Within the determination range, puerarin and daidzin control substances showed good linear relationship. And The puerarin and daiazin contents in total flavonoids of RADIX PUERARIAE were 5.41 and 7.50 μg/ml, respectively. [Conclusion] This research provided basis for better utilization of RADIX PUERARIAE.
2011年06期 v.2 45-46页 [查看摘要][在线阅读][下载 129K] -
[Objective] To establish the fingerprint of chemical constituents in ethyl acetate parts by HPLC, so as to provide a basis for quality control and variety identification of SEMEN PLANTAGINIS. [Method] The chromatographic separation was performed on a C18 column (250 mm×4.6 mm, 5 μm) using the mixture of acetonitrile-0.5% acetic acid aqueous solution as mobile phase in gradient elution. The flow rate was 1.0 ml/min, and the detection wavelength was 270 nm. [Result] There were 12 common peaks in the HPLC fingerprint of chemical constituents in ethyl acetate parts of 10 batches of SEMEN PLANTAGINI produced in Jiangxi authentic regions. Fingerprint similarity of ethyl acetate parts of SEMEN PLANTAGINI was varied obviously for different varieties. [Conclusion] The HPLC fingerprint method was simple, rapid and feasible in the quality control and variety identification of SEMEN PLANTAGINI.
2011年06期 v.2 47-50页 [查看摘要][在线阅读][下载 279K] - Maynur Kadir;Balati Kuerban-jiang;
[Objective] This study was to investigate the fat and protein components in seeds of wild Elaeagnus angustifolia from Xinjiang Uigur Autonomous Region. [Method] GC-MS was employed to measure the content and composition of fatty acids in the seeds of E. angustifolia, and fractional extraction method to isolate protein and semi micro-quantitative nitrogen method to determine amino acid components. [Result] Fat content in E. angustifolia seeds was 8.20% (with unsaturated fatty acids accounting for the dominant proportion), of which the fatty acid composition was in order linoleic acid (46.52%), oleic acid (33.17%), linolenic acid (9.21%) and stearic acid (5.31%). Mean content of protein in E. angustifolia seeds was 11.31%, of which globulin and albumin account for 70%, while the contents of residue protein, gliadin and glutenin were lower. Eighteen kinds of amino acids were found in the proteins of E. angustifolia seeds, of which Asp content was the highest (12.70%), followed by Glu (11.80%), Tyr (9.33%), Leu (8.11%), Lys (7.22%), Arg, Ser, Ala, Val, His, Met and Cys. In detail, the content of essential amino acids of human body reached 28.76%. [Conclusion] Seeds of E. angustifolia from Xinjiang Uigur Autonomous Region are full of nutrients, having a promising future.
2011年06期 v.2 51-53页 [查看摘要][在线阅读][下载 90K] -
[Objective] The aim was to establish a method to determine the contents of trace elements in the wild and planted Rhodiola. [Method] The inductively coupled plasma mass spectrometer (ICP-MS) was adopted to determine and analyze several trace elements in the wild and planted Rhodiola. [Result] Under the selected experimental conditions, the detection limit was 0.002-0.092 ng/ml, the relative standard deviation (RSD) was between 1.87% and 4.96%, and the recovery rate of standard addition was 94.0%-104.5%. [Conclusion] The gross content of trace elements was higher in the wild Rhodiola than in the planted Rhodiola, but the contents of harmful elements such as Pb, As and Cd in wild Rhodiola were lower.
2011年06期 v.2 54-56页 [查看摘要][在线阅读][下载 85K] [Objective] The aim was to establish an easy and rapid method to determine the content of five trace elements in Arundina graminifolia (D.Don)Hochr.. [Method] The contents and dissolution rates of Ca, Cu, Fe, Pd and Zn in A. graminifolia were determined by flame atomic adsorption spectrometry after normal digestion. [Result] A. graminifolia had abundant contents of Ca, Fe and Zn, which were 919.80, 361.10 and 39.89 μg/g, respectively; but the dissolution rates of Ca and Fe from the decoction were very low after water boiling. [Conclusion] The digestion method with platen heater had the advantages of simple equipment, convenient operation, complete digestion, high precision, and good accuracy. Therefore, it was one of the best methods to determine the trace elements.
2011年06期 v.2 57-58+61页 [查看摘要][在线阅读][下载 128K] -
[Objective] The aim as to analyze the chemical components of volatile oil from Artemisia Lactiflora Wall in north Guizhou Province of China. [Method] Steam distillation was used to extract the volatile oil from A. lactiflora. GC-MS coupling technique was adopted to analyze the chemical components of volatile oil. [Result] A total of 64 components were isolated and identified from the volatile oil of A. lactiflora in north Guizhou Province, which accounted for 94.662% of the total number of volatile oils. Among them, terpenoid was the most abundant. (-)-Lavandulol had the greatest content, accounting for 17.781%; and Germacrene D accounted for 11.963%. [Conclusion] A. lactiflora in north Guizhou had more abundant volatile oils than that in Guangdong. (E)-β-Farnesene, Nerolidol, Spathulenol, Caryophyllene oxide, and Zingiberene were all found in A. lactiflora in the two places; and contents of Spathulenol and Caryophyllene oxide in A. lactiflora in Guangdong were greatly different from those in north Guizhou Province.
2011年06期 v.2 59-61页 [查看摘要][在线阅读][下载 122K] - ABULIMITI Yili;HAJIAKBER Aisa;
[Objective] To study the chemical compositions and biological characteristics of essential oil from Cuminum cyminum L. planted in Hotan of Xinjiang Province. [Method] The essential oil was firstly extracted from seeds of Cuminum cyminum L. by steam distillation method, and then its chemical compositions were analyzed and identified by gas chromatography-mss spectrometry (GC-MS) and standard spectrum library. [Result] 48 chromatographic peaks were isolated from seeds of Cuminum cyminum L., of which 38 compounds that accounted for 98.55% of the total essential oil were identified. The most abundant compounds were cuminal (39.03%), 1-methyl-2-isopropylbenzene (12.85%), 3-caren-10-al (9.15%) and 4-ethyl-3-nonen-5-yne (8.28%). The Cuminum cyminum L. essential oil showed good antibacterial, sterilization and antioxidant activity. The MIC of essential oil against Staphylococcus aureus and Escherichia coli both were 1.25 μl/ml. [Conclusion] The study could provide a scientific basis for further exploration and utilization of Cuminum cyminum L. planted in Hotan of Xinjiang Province.
2011年06期 v.2 62-64+67页 [查看摘要][在线阅读][下载 137K] -
[Objective] To study karyotype analysis of Lilium leucanthum (Baker) Baker in Shennongjia national nature reserve, so as to supply some cytological data for studying genetic diversity, origin and systematical evolution of Lilium leucanthum (Baker) Baker. [Method] Karyotype of Lilium leucanthum (Baker) Baker was analyzed by the conventional section method. [Result]The chromosome number was 2n=2x=24, and the basic chromosome number was x=12. Karyotype formula was K(2n)=24=4m (2SAT)+2sm +8st+10t, mainly consisting of terminal and subterminal chromosomes. Chromosome composition of relative length was 2n=24=4L+4M2+14M1+2S, whose karyotype asymmetric coefficient was 79.66% belonging to "3B" type. [Conclusion] The karyotype of Lilium leucanthum (Baker) Baker in Shennongjia national nature reserve belong to evolutionary type in systematic and evolutionary of family Liliaceae.
2011年06期 v.2 65-67页 [查看摘要][在线阅读][下载 198K] 下载本期数据