- Yunzhang XU;Xiaoyan ZHAO;Jingxia WANG;Mei SUN;Yuan LIU;Jizhong ZHANG;
[Objective]To compare the content of moisture,ash and extract of stem and root bark of Eucommia ulmoides Oliver and Euonymus grandiflorus Wall.f.grandiflorus.[Methods]According to the first method of aquametry(over-drying method)in the 2010 edition Chinese Pharmacopoeia appendix IX H,ash determination in appendix IX K,and extract measurement in appendix X A.[Results]Moisture content of stem and root bark of Euonymus grandiflorus Wall.f.grandiflorus was between 5.38%and 6.33%,while that of Eucommia ulmoides Oliver was between 5.36%and 6.90%(both in line with the stipulation in Chinese Pharmacopoeia that moisture content of Eucommia ulmoides Oliver herbs should at most be 13.0%);total ash content of stem and root bark of Euonymus grandiflorus Wall.f.grandiflorus was between15.88%to 19.43%,while that of Eucommia ulmoides Oliver was between 5.45%and 7.30%(The average content of Eucommia ulmoides Oliver was 6.29%,and it was in line with the stipulation in Chinese Pharmacopoeia that of total ash content of Eucommia ulmoides Oliver should at most be 10%;and the average content of stem and root bark of Euonymus grandiflorus Wall.f.grandiflorus was 17.36%,and it was higher than the stipulated level.);the acid-insoluble ash content of stem and root bark of Euonymus grandiflorus Wall.f.grandiflorus was between 1.88%and 2.64%,lower than that of Eucommia ulmoides Oliver(3.58%-4.10%);the content of 75%hot ethanol extract of Euonymus grandiflorus Wall.f.grandiflorus was the highest of 26.51%;and under the extract condition,the content of stem and root bark of Euonymus grandiflorus Wall.f.grandiflorus was between 19.36%and 26.45%,obviously higher than that of Eucommia ulmoides Oliver(14.96%-16.70%)(Both of that were in line with the stipulation in Chinese Pharmacopoeia,and the ethanol-soluble extract content of stem and root bark of Eucommia ulmoides Oliver should be at least 12.0%);there was little difference in ether volatile extract content of Euonymus grandiflorus Wall.f.grandiflorus and Eucommia ulmoides Oliver,respectively in the range of 0.33%-0.51%and 0.33%-0.56%.[Conclusions]The appropriate moisture content of Euonymus grandiflorus Wall.f.grandiflorus should not exceed 13%.Euonymus grandiflorus Wall.f.grandiflorus are widely used by of Yi nationality clinic,however it was few research on chemical components,bioactivity and the action mechanism of toxicology and pharmacology.Moreover results of this experiment showed that the content of ethanol-soluble extract of Euonymus grandiflorus Wall.f.grandiflorus was higher than that of Eucommia ulmoides Oliver by hot 75%ethanol extraction,while content of ether volatile extract in the two materials were similar.Therefore,bio-screening of main constituents and relevant researches on effects of toxicology and pharmacology should be done to testify and ensure the safety and validity of Yi Nationality clinic,developed and medical used scientifically and properly.
2014年03期 v.5 19-22+25页 [查看摘要][在线阅读][下载 155K] - Zhengming YANG;Bo LI;Panpan LIU;Yijun CHEN;Yuan LIU;
[Objective]To establish a systematic preliminary research on the chemical composition of Artemisia vestitaWall.[Methods]Systematic preliminary tests was adopted;physical and chemical identification of the water extracted solutions,acidic ethanol extracted solutions and petroleum ether extracted solutions were carried out for A.vestita Wall.,respectively.The chemical components were preliminarily identified.[Results]A.vestita Wall.had saponins,tannins,organic acids,phenols,flavonoids,anthraquinones,coumarin,lactones,alkaloids,steroids or triterpene,volatile oil,grease and etc.[Conclusions]It has been preliminarily confirmed that A.vestita Wall.contained various chemical components,which provided an experimental data base for further research and development of A.vestita Wall.
2014年03期 v.5 23-25页 [查看摘要][在线阅读][下载 124K] - Yuqing HUANG;Xiaoxue LU;Dewei ZHANG;Suoyi HUANG;Rong LI;
[Objective]In order to take full advantage of the source of leaves of Jasminum samba and supply raw materials for all kinds of new production of pigments such as edibles and medical drug,the microwave-assisted method was utilized for the deeper research on the extraction of pigments of leaves of Jasminum samba.[Methods]By using single factor experiment and orthogonal experiment,the optimal ultrasonic extraction process of pigments of leaves of Jasminum samba was achieved.[Results]The optimal ultrasonic extracting condition:the time of ultrasonic extraction was 9 min,solid-to-liquid ratio was 1∶20.The power of ultrasonic was 120 W.[Conclusions]By the use of this simple,handy method,the optimal ultrasonic extraction process was discovered.
2014年03期 v.5 26-28页 [查看摘要][在线阅读][下载 334K] - Ying ZHU;Dijia YU;
[Objective]To identify the chemical components in the volatile oils of FRUCTUS TSAOKO in Yunnan Province.[Methods]Volatile oils were extracted from FRUCTUS TSAOKO by steam distillation method.Volatile oils in FRUCTUS TSAOKO were analyzed and identified by GC-MS method.Relative percentage of each component was determined by normalization method.HP-5MS capillary column(0.25 mm×30 m,0.25μm)was adopted.The temperature of injection port was 250℃;column temperature was 40℃(kept for 2 min)→150℃(kept for 2 min),10℃/min,150→250℃(kept for 5 min),15℃/min.Split ratio was 10∶1;and flow rate was 1.0 mL/min.[Results]A total of 37 chemical components were isolated and identified.Among them,the main components were eucalyptol(14.58%),3,7-dimethyl-2,6-diene aldehyde(6.66%),α,α,4-3-methyl-3-cyclohexene-1-methanol(8.49%),α-pinene(5.65%),β-pinene(4.54%)and so on.[Conclusions]This method had good isolation effects and could be used for the quality evaluation of FRUCTUS TSAOKO.
2014年03期 v.5 29-30+33页 [查看摘要][在线阅读][下载 239K] - Zhuo HAN;Xiaoyan CHEN;Yanhong LI;Hanju SUN;
[Objective]To study the volatile constituents from the leaves of Rubus chingii Hu.[Methods]The volatile constituents of R.chingii leaves were analyzed by HS-GC/MS.The constituents were identified by mass spectra using NIST.The relative percentage content of volatile constituents was calculated by the relative peak area method.[Results]A total of 37 constituents were isolated and identified.Among them,hexadecanoic acid had the maximum content.[Conclusions]The volatile constituents of R.chingii leaves were isolated and analyzed by HS-GC/MS,which provided scientific references for the development and utilization of R.chingii leaves.
2014年03期 v.5 31-33页 [查看摘要][在线阅读][下载 186K] - Jinqing LU;Qiang LI;Shengnan GUO;Junlong CAI;Xiaoshuang LI;Yi DAI;Huan LIANG;Xiaojin YANG;
[Objective]To establish the HPLC fingerprint of flavonoids from SEMEN TRIGONELLAE extracts.[Methods]ZORBAX SB-C18chromatographic column(4.6 mm×250 mm,5μm)was adopted;based on gradient elution,mobile phase was methanol-0.4%phosphoric acid solution.Flow rate was 0.8 mL/min;detection wavelength was 339 nm;and injection volume was 10μL.A total of 10 batches of SEMEN TRIGONELLAE extracts were measured by HPLC with vitexin as the reference substances.[Results]The common mode of the HPLC fingerprints was established;and a total of 11 common peaks were marked.The similarity degrees of the 10 batches of samples were all greater than0.9.[Conclusions]This method was simple,accurate and repeatable,and provided scientific basis for the quality control standard and quality evaluation of SEMEN TRIGONELLAE extracts.
2014年03期 v.5 34-36+40页 [查看摘要][在线阅读][下载 353K] - Xiaoyan ZHAO;Jingxia WANG;Yunzhang XU;Yuan LIU;Jizhong ZHANG;
[Objective]To establish the simultaneous detection method for four index components in Banxia Xiexin Decoction by RP-HPLC,which were baicalin,glycyrrhizic acid,glycyrrhetinic acid and oleanolic acid.[Methods]Diamonsil C18(2)chromatographic column(4.6 mm×250 mm,5μm)was adopted.Mobile phase was methanol(A)-0.5%phosphoric acid aqueous solution(B)with gradient elution;flow rate was 1.0 mL/min;column temperature was 30℃;detection wavelength was 230 nm,and injection volume was 20μL.[Results]The detection limits of baicalin,glycyrrhizic acid,glycyrrhetinic acid and oleanolic acid were 0.031,0.025,0.004 and 0.007μg,respectively.And the baicalin,glycyrrhizic acid,glycyrrhetinic acid and oleanolic acid all reached good isolation.Their linear ranges were0.330-16.50μg(r=0.999 5),0.347-17.37μg(r=0.999 0),0.035-1.74μg(r=0.998 4)and 0.027-1.330μg(r=0.999 3);and the average recovery rates were 101.4%(RSD=1.36%),98.5%(RSD=1.07%),96.9%(RSD=0.84%)and 99.0%(RSD=1.79%).[Conclusions]This method was simple,stable,precise and practical,and could be used for the simultaneous detection of four index components in Banxia Xiexin Decoction.
2014年03期 v.5 41-44页 [查看摘要][在线阅读][下载 269K] - Jingxia WANG;Mei SUN;Zhengming YANG;Bo LI;Jizhong ZHANG;Yuan LIU;
[Objective]To carry out the systematic preliminary test on the chemical components of RADIX at RHIZOMA CYATHULAE CAPITATAE,and to compare with RADIX et RHIZOMA CYATHULAE OFFICINALIS and RADIX et RHIZOMA ACHYRANTHIS BIDENTATAE.[Methods]Systematic preliminary tests was adopted;physical and chemical identification of the water extracted solutions,ethanol extracted solutions and petroleum ether extracted solutions were carried out for RADIX at RHIZOMA CYATHULAE CAPITATAE,RADIX et RHIZOMA CYATHULAE OFFICINALIS and RADIX et RHIZOMA ACHYRANTHIS BIDENTATAE,respectively.The chemical components were preliminarily identified and compared.[Results]The three medicines all had saccharides,glycosides,proteins,saponins,phenols,organic acids,flavonoids,alkaloids,sterides,triterpenes and so on.Hydrochloric acid-magnesium powder test on the identification of flavonoids showed that the three medicines were all negative,indicating that the structures of flavonoids components were similar.Alkali test on the anthraquinones identification showed that only RADIX at RHIZOMA CYATHULAE CAPITATAE was negative;identification test on volatile oil and grease showed that only RADIX et RHIZOMA ACHYRANTHIS BIDENTATAE was positive.[Conclusions]It was preliminarily identified that there were saccharides,glycosides,proteins,saponins,phenols,organic acids,flavonoids,alkaloids,sterides and triterpenes in RADIX at RHIZOMA CYATHULAE CAPITATAE.
2014年03期 v.5 45-47页 [查看摘要][在线阅读][下载 119K] - Yazhong ZHANG;Zhen ZONG;Yongsheng BAN;Jie YUAN;Lidan HUANG;Xiaomei XIE;Bin JIN;
[Objective]In order to control the quality of roots of Stemona sessilifolia(Miq.)Miq.,we established the standard of microscopic identification,thin-layer identification,moisture content,total ash,acid-insoluble ash,extractum and content determination.[Methods]Transverse section and powder were used in microscopic identification.Thin-layer identification of alkaloids was performed by TLC.Moisture content,total ash,acid-insoluble ash and extractum were determined by methods described in appendix of Chinese Pharmacopoeia.The content of protostemonine was measured by HPLC.Chromatography conditions.Phenomenex Gemini C6-phenyl.column(250 mm×4.6 mm,5μm);mobile phase:mix of acetonitrile and 0.1%triethylamine(34∶66,V/V);detection wavelength 306 nm;column temperature:30℃;flow rate:1.0 mL/min.[Results]Microscopic features of transverse section and powder were accordant with those recorded in Chinese Pharmacopoeia and documents.The number of phloem bundles and xylem bundles in stele differed.The chromatographic spot of thin-layer identification was very clear which indicated a good separation.Protostemonine displayed a good linear relation(R=0.999 8)when its concentration was6.257-156.42 mg/L.The average recovery rate reached 100.2%,(RSD 1.63%,n=6).[Conclusions]The method was simple to operate,accurate and had a good repeatability.It could be used to control the quality roots of Stemona sessilifolia(Miq.)Miq.
2014年03期 v.5 48-52+56页 [查看摘要][在线阅读][下载 1180K]